The UV-method for the measurement of serum transaminsases was critically evaluated, with respect to condition of storage of reagents, grade of coupling enzymes, presence of cofactor, and absorbance linearity of instrument.
Those factors gave an obvious influence on the results of UV-procedure separately or in combination.
Above findings lead to the conclusion that the standard procedure for any enzyme analyses should be presented with the accummulation of the ditailed data on all of the assay conditions.