This study was conducted to evaluate the digestion efficiency on anaerobic digestion of livestock wastewater and food wastes using hybrid in low temperature. Anaerobic digester was operated from 30 to 60 day's HRT at the temperature of 25°C. The organic removal efficiency was around 60% of TCOD and more than 70% of SCOD. In case HRT 30 and 40, total gas production was over 3000 mL/day and methane gas production was over 2000 mL/day. In case HRT 50 and 60, total gas production was over 1800 mL/day and methane gas production was over 1000 mL/day. So gas production of HRT 30 and 40's rector was about 2 times than HRT 50 and 60.