Dataset for high-throughput proteomics by DIA using Q-Orbitrap mass spectrometer and 5-min gradient LC
2023 Volume 5 Pages 7-
Details
2023 Volume 5 Pages 7-
To apply MS-based proteomics for medical and medicinal applications, it is necessary to improve the analytical throughput based on the requirement of analyzing large number of samples. We precisely evaluated DIA parameters for 5-min gradient LC and reached a depth of >5,000 proteins from 1,000 ng HEK293T cell digest in a single-shot run, with an analytical throughput of 80 samples/day1.
