The striatum plays an important role in linking cortical activity to basal ganglia outputs. We conducted the Ca2+ imaging study to investigate the spontaneous activities of the striatum. Corticostriatal slices of 10-25 days old Sprague Dawley rats were stained with Fura-PE3-AM to measure intracellular Ca2+ concentration ([Ca2+]i) using a cooled-CCD imaging system. Long lasting spontaneous [Ca2+]i transients, which lasted up to about 250 s, were observed. Most cells exhibited irregular frequencies, but some exhibited oscillatory feature. A pairwise correlation analysis revealed that some cells appear to belong to a correlated network. Administration of TTX or of CNQX + AP5 did not block the [Ca2+]i transients. Therefore, the action potentials and the excitatory synaptic inputs in the striatal network were not involved in induction of the [Ca2+]i transients. In contrast, the number of active cells, which exhibited the [Ca2+]i transients, was greatly reduced by the intracellular Ca2+ store depletor, thapsigargin, and was reduced little by the administration of the Ca2+-free saline. Therefore, the intracellular Ca2+ store is likely to contribute to the [Ca2+]i transients. In the mouse, which expressed green fluorescent protein (GFP) in astrocytes (GFAP-GFP mouse), both the GFP positive cells and the GFP negative cells exhibited the [Ca2+]i transients. These results suggested that both astrocytes and neurons may exhibit the long lasting spontaneous [Ca2+]i transients in the striatum. [J Physiol Sci. 2006;56 Suppl:S83]