Objective: The aim of this in vitro study was to determine the cytotoxicity of 3 different soft and hard lining materials which is used in prosthodontic clinic. Material and methods. Meliodent (Bayer Dental, Germany) , Viscogel (dentsply.uk) and Molloplast (Detax Gmbh & Co., KG, Germany) were evaluated with MA-104, Vero and primary tooth root cell culture for 1,3 and 8 days. Results. The results showed that the eluates from Viscogel was not cytotoxic, Molloplast and was cytotoxic(20%) on primary tooth root cell culture, On the other hand Viscogel was cytotoxic(10%), Molloplast was not cytotoxic on MA-104 cell culture. Viscogel(35%) was also found cytotoxic on Vero cell culture. Meliodent was not cytotoxic on all cell cultures Conclusion. The influence of the cytotoxicity depended on the materials and type of cell culture tested. However, additional in vivo studies are needed to evaluate the potential toxicities of the prosthodontic dental materials during clinical operative procedures.