A protective role for type I interferon signaling following infection with Mycobacterium tuberculosis carrying the rifampicin drug resistance-conferring RpoB mutation H445Y

doi:10.1371/journal.ppat.1012137

A protective role for type I interferon signaling following infection with Mycobacterium tuberculosis carrying the rifampicin drug resistance-conferring RpoB mutation H445Y

Fig 2

Type I IFN signaling signatures are common across wt and rpoB-H445Y Mtb infections.

C57Bl/6 (B6), Ifnar1^-/-, Il1r1^-/-, and Ifnar^-/- Il1r1^-/- [double knockout (DKO)] mice were aerosol infected with a low dose of wt or rpoB-H445Y Mtb and sacrificed at 14 dpi. RNA was extracted from homogenized lung tissue and sequenced. Heatmaps displaying average expression levels of top differentially expressed genes (DEGs) across mouse genotypes after A) wt or B) rpoB-H445Y Mtb infection with annotated pathways (four to six mice per group). Significant DEGs related to the corresponding reactome pathways are visualized using heatmaps(|log₂FC| >0, adjusted p-value < 0.05). Average expression (normalized) of selected genes on log₂ scale are shown.

doi: https://doi.org/10.1371/journal.ppat.1012137.g002