BAG6 inhibits influenza A virus replication by inducing viral polymerase subunit PB2 degradation and perturbing RdRp complex assembly
Fig 1
The overexpression of BAG6 inhibits IAV replication.
(A and B) A549 cells were transfected with BAG6-HA or Empty vector (EV) plasmids. At 24 h post-transfection, the cells were infected with IAV H1N1 (MOI = 1.0). The viral NP and M1 expression were measured by western blotting at different time points postinfection, as indicated (A, left panels), and densitometry analysis and quantification were performed (A, right panels). Viral titers in the supernatants were determined by TCID50 assay at 12, 24 and 36 h post-infection (B). (C-H) A549 cells were transfected with BAG6-HA or Empty vector (EV) plasmids. At 24 h post-transfection, the cells were infected with IAV H7N9 (C), H9N2 (E) and H5N1 (G) (MOI = 1.0). The viral NP expression was measured by western blotting at 12 and 24 h post-infection, as indicated (left panels), and densitometry analysis and quantification were performed (right panels). Viral titers in the supernatants were determined by TCID50 assay at 12 and 24 h post-infection with H7N9 (D), H9N2 (F) and H5N1 (H). Data presented as means ± SD and are representative of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, Unpaired Student’s t test.