Influenza virus infection enhances tumour-specific CD8+ T-cell immunity, facilitating tumour control
Fig 2
CD8+ T-cells are essential for tumour growth suppression during IAV infection.
Tumours were subcutaneously (s.c.) transplanted and mice were infected with 150 PFU/mL Influenza A/PR/8/34 (IAV) as described. (a) 200 μg anti-IFNAR antibody was applied intraperitoneally (i.p.) on days 4, 7 and 10. n = 6. (b) For Natural Killer (NK)-cell or macrophage depletion 200 μg of antibodies against NK1.1 or CSF1R were applied i.p. respectively on days 4, 7 and 10. n = 3. (c) Rag2-/- or BALB/c wildtype mice were s.c. injected with 1 x 105 CT26 tumour cells followed by IAV (150 PFU/mL) infection. Data from 2 experiments with 3–4 mice per group per experiment. (d) For selective T-cell depletion 200 μg of antibodies against CD4 or CD8 were applied i.p. on days 4, 7 and 10. Data from 2 experiments with 4 mice per group per experiment. Error bars represent SEM. Statistical test on tumour growth development was performed as Two-way-ANOVA followed by Tukey’s multiple comparisons test. ** = p<0.01, **** = p<0.0001.