Virus specificity and nucleoporin requirements for MX2 activity are affected by GTPase function and capsid-CypA interactions
Fig 4
Phosphorylation at residue T151 does not determine CypA-independent MX2 activity.
A) Infection HT1080 cells stably transduced with doxycycline-inducible myc-tagged MX2 or MX2T151D in the presence (open bars) or absence (filled bars) of doxycycline and presence or absence of CsA with the indicated GFP reporter viruses. Titers are represented as mean + sem of infectious units (IU) per pg of reverse transcriptase (RT), n≥9 technical replicates combined from three-five independent experiments. Statistical analysis in S1 File. B) Western blot analysis of doxycycline-inducible MX2-myc and tubulin loading control.