The hybrid RAVE complex plays V-ATPase-dependent and -independent pathobiological roles in Cryptococcus neoformans
Fig 6
Phenotypic analysis of pH-dependent or -independent function of the hRAVE complex.
(A) Qualitative spot assays under various temperatures (25°C, 37°C, and 39°C) and pH levels (pH 5.5, pH 6.8, and pH 7.5). (B) Quantitative growth rates of the WT and rav1Δ strains on different pH levels. Growth was monitored at 30°C by measuring OD600 with a multi-channel bioreactor for 50 h. Each curve represents a single trial of two independent experiments. (C) Qualitative spot assays under indicated stress conditions at different pH levels. Plates were incubated at 30°C for 3 days. Abbreviations: TBH (tert-butyl hydroperoxide), 0.7 mM; MD (menadione), 0.02 mM; MMS (methyl methanesulphonate), 0.03%; HU (hydroxyurea), 100 mM; CR (Congo red), 0.8%; CFW (calcofluor white), 5 mg/ml; CDS (cadmium sulfate), 25 μM; AMB (amphotericin B), 1.6 μg/ml; H2O2 (hydrogen peroxide), 3 mM; DIA (diamide), 2.5 mM; TM (tunicamycin), 0.3 μg/ml; DTT (dithiothreitol), 16 mM; SDS (sodium dodecyl sulfate), 0.03%; SBR (YPD + 2 M sorbitol); FCZ (fluconazole), 10 μg/ml; FDX (fludioxonil), 1 μg/ml; 5FC (5-flucytosine), 300 μg/ml. Blue and red fonts indicate pH-dependent and -independent phenotypes, respectively.