Bcl-xL is required to protect endothelial cells latently infected with KSHV from virus induced intrinsic apoptosis
Fig 6
Expression of KSHV latent locus is sufficient to render Bcl-xL necessary for survival.
TIME cells were transduced and selected for sgRNAs to Bcl-xL or non-targeting control and infected with either a control gutted adenovirus expressing only GFP (ad-GFP) or a gutted adenovirus virus expressing only the KSHV latent locus (KLAR). 48 hpi cell viability was measured using a trypan blue assay. Data are presented as mean ± s.d. (2-way ANOVA, n = 4) *P<0.05.