Identification of diphenylurea derivatives as novel endocytosis inhibitors that demonstrate broad-spectrum activity against SARS-CoV-2 and influenza A virus both in vitro and in vivo
Fig 2
DPUDs exhibit high selectivity indices against IAV and SARS-CoV-2 infection.
(A-C) Graphs showing concentration-dependent effect of DPUDs against IAV (X-31 and WSN) and SARS-CoV-2 (D614G) infections in A549 and Vero-E6 cells, respectively. The half-maximal inhibitory concentration (IC50) values corresponding to each compound are shown. (D-E) Cytotoxicity assessment DPUDs in A549 and Vero-E6 cells. The cells were treated with different concentrations of DPUDs for 24 h, following which, LDH cytotoxicity assay was performed. The 50% cytotoxic concentration (CC50) values corresponding to each compound are shown. (F) Selectivity indices of the compounds for IAV X-31 and WSN, and SARS-CoV-2 (D614G) infections.