Reinforcement amid genetic diversity in the Candida albicans biofilm regulatory network
Fig 8
Biofilm formation dependence on WOR3.
Panels A and B. Biofilm formation ability for WT, wor3Δ/Δ, and complemented strains was assessed at 37°C for 24 hrs in RPMI, RPMI+10% FBS, and YPD+10% FBS in both SC5314 (Panel A) and P87 (Panel B) backgrounds using the “96 well plate–Fluorescence microscopy” method. Side-view images of two independent mutant strains were tested for each clinical isolate background for both the wor3Δ/Δ and complemented strains. Scale bars are equal to 285 μm. Panel C. SC5314-derived wild-type, mutant, and complemented strains were tested for biofilm formation in a rat venous catheter infection model [21]. C. albicans cell counts per catheter were determined at 48 hr post-infection. Wild-type and mutant strains differed with p-value = 0.02.