Mitochondrial protein, TBRG4, modulates KSHV and EBV reactivation from latency
Fig 6
Knockdown of TBRG4 also induces EBV lytic reactivation.
(A) AGS-EBV cells were treated with MitoTracker to visualize the mitochondria (blue), then fixed and stained with TBRG4 antibody (red). AGS-EBV cells contain a recombinant EBV constitutively expressing GFP (green). Images were taken using Leica DMi8. (B) AGS-EBV cells were transfected with control NS or TBRG4 siRNA for 48 h and then treated with TPA for 24 h. EBV genome copies were measured by real-time PCR in the supernatant from vehicle- and TPA-treated cells. (C-G) RNA was extracted from the AGS-EBV cells treated as in (B), and the knockdown efficiency of TBRG4 and the mRNA expression of EBV genes LMP1, BMRF1, BALF2, BLLF1 were measured by real-time PCR. (H) Cell lysates were harvested from the AGS-EBV cells treated as in (B), and western blots were performed with the indicated antibodies. The data shown are representative of three independent experiments. Data are presented as mean ± SD. *, P<0.05 by student’s t-test.