Expanding the tolerance of segmented Influenza A Virus genome using a balance compensation strategy
Fig 6
Establishment of an in vivo imaging mouse model of IAV subtype H3N2 infection.
(A) Comparation of the in vitro replication kinetics of wildtype X31 and reporter X31-NSCE2-Fluc viruses. (B) The lethality of wildtype X31 and reporter X31-NSCE2-Fluc viruses in mouse models. (C) Mice were infected with series doses of X31-NSCE2-Fluc virus and the bioluminescence was monitored at days 1, 2, 3, 5, and 8 post challenge. (D) The kinetics of bioluminescence imaging in mice infected with varies doses of X31-NSCE2-Fluc virus. Inset, linearization analysis of the bioluminescence signals at day 2 post challenge to challenge doses.