Bet-hedging antimicrobial strategies in macrophage phagosome acidification drive the dynamics of Cryptococcus neoformans intracellular escape mechanisms
Fig 4
Comparisons of labelled macrophage populations and the proportion of which have ingested C. neoformans 24 hours post infection.
A. The uncorrected proportion of adherent macrophages containing C. neoformans at the end of the infection. Data was collected from 10 independent experiments. B. The proportion of macrophages containing yeast after correcting for floating cells. Both the proportion of labelled cells compared to the original population of infected M1 macrophages as well as the frequency of labelled cells which ingested C. neoformans remained consistent throughout the time course. *, *** signify P < 0.05 and 0.001 via ANOVA with Tukey multiple comparisons. C. A model depicting expected proportions of infected cells based only on random chance after sequential Dragotcytosis events for M0 (light gray), M1 (dark gray), and M2 (black) macrophages. Boxplots signify median with 95% confidence interval tails.