The glycerol-3-phosphate dehydrogenases GpsA and GlpD constitute the oxidoreductive metabolic linchpin for Lyme disease spirochete host infectivity and persistence in the tick
Fig 5
GpsA regulates central metabolism and redox balance.
(A) Polar metabolomics of bacterial strains subjected to principal component (PC) analysis with pareto-scaling. (B) The corresponding metabolite loading distribution for the analysis displayed in (A) for principal components 1 and 2. (C) Metabolites that significantly vary between ΔgpsA and wild type (WT) with a false discovery rate (FDR) less than 5%. Values in the heatmap at left are displayed as the log2(fold change mutant versus WT) and values in the accompanying heatmap at right indicate whether that metabolite passes a 5% FDR filter for the indicated comparison as assessed by a Benjamini-Hochberg correction. (D) Metabolic map of the changes in glycolysis and the glycerol shunt that occur with the loss of GpsA. All measured metabolites in the included pathways are displayed. The log2(fold change ΔgpsA versus WT) is displayed as color of the node and the -log(p-value) is displayed as the size of the node. Enzymes in the glycerol arm of metabolism are displayed as diamonds. Data are from four independent biological replicates.