Absence of signal peptide peptidase in peripheral sensory neurons affects latency-reactivation in HSV-1 ocularly infected mice
Fig 4
Expression of gB, gK, and ICP0 transcripts in TG of Avil-SPP-/- infected mice.
Avil-SPP-/- and control mice were ocularly infected with 2 X 105 PFU/eye of HSV-1 strain McKrae. TG were harvested on days 2 and 5 PI. RNA from individual TG or from the neuron fraction of four combined TG were isolated as described in Materials and Methods. gB, gK and ICP0 transcripts were measured by qRT-PCR and copy numbers of each transcript were calculated using standard curves generated using their respective plasmids. Neuronal RNA was isolated from four combined TG, while total TG RNA was isolated from individual TG. Each bar represents mean ± SEM from 12 mouse TG for neurons (A and C), and 12 TG (B and D) from six mice for total TG RNA.