Coordinated action of multiple transporters in the acquisition of essential cationic amino acids by the intracellular parasite Toxoplasma gondii
Fig 6
TgApiAT6-1 and TgApiAT1 are net accumulators of cationic amino acids.
A-C. Time-course measuring the Lys (A) or Arg (B-C) concentration in TgApiAT6-1 expressing oocytes (A-B; squares), TgApiAT1 expressing oocytes (C; squares), or H2O-injected oocytes (A-C; circles) incubated in 1 mM Lys (A) or 1 mM Arg (B-C) as quantified by LC-MS/MS. Following 32–34 hr of incubation measuring accumulation inside oocytes, samples were split into two groups, one continuing with substrate incubation (closed symbols), the other with substrate replaced by substrate-free incubation media (open symbols). Each data point represents the mean intracellular amino acid concentration ± S.D. of 12 individual oocytes (substrate-incubated) or 3 batches containing 5 oocytes each (substrate-free), and are representative of 3 independent experiments.