Substrate-mediated regulation of the arginine transporter of Toxoplasma gondii
Fig 3
TgApiAT1 regulation is mediated by a range of nutrients.
(A, D, F, H) Western blots of TgApiAT1-HA3 in parasites grown at a range of (A) [Lys], (D) [Tyr], (F) [Gln], or (H) [D-glucose] in the growth medium. TgTom40 is a loading control. Data are representative of three independent experiments. (B) nanoLUC:fLUC ratio in parasites grown in media containing a range of concentrations of Lys. Data represent the mean ± SD from three independent experiments. P values were calculated using a one-way ANOVA with Tukey’s multiple comparisons test. P values not shown were > 0.40. (C) nanoLUC:fLUC ratios in parasites grown in media containing a range of [Arg] and either 50 μM Lys (light grey) or 1 mM Lys (dark grey). Data represent the mean ± SD from three independent experiments. P values were calculated using unpaired t-tests, not assuming equal variance (d.f. = 4). (E, G, I) nanoLUC:fLUC ratios in parasites grown in media containing a range of concentrations of (E) Tyr, (G) Gln, or (I) D-glucose. Data represent the mean ± SD from three independent experiments, with the ratios normalised to the condition with the highest nutrient concentration. P values were calculated using a one-way ANOVA with Dunnett’s multiple comparisons test, comparing the normalised nanoLUC:fLUC ratios at each nutrient concentration to the condition containing the highest concentration tested. P values not shown were > 0.50.