Pore-forming alpha-hemolysin efficiently improves the immunogenicity and protective efficacy of protein antigens
Fig 2
Antigen-positive cells in muscles and draining lymph nodes (LNs).
(A) Alexa Fluor 488-labeled antigen uptake in the muscle and draining LNs 24 and 72 hours after injection with His buffer, PA0833, HPF, PA0833 + Alum, or HPF + Alum (n = 5). Antigen-positive cells in the muscles (B) and draining LNs (inguinal lymph nodes) (C) were detected using a flow cytometer 24 h and 72 h after immunization. Data are shown as the mean ± SEM. Two-way ANOVA, Tukey’s multiple comparison test, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.