Transgenic expression of a T cell epitope in Strongyloides ratti reveals that helminth-specific CD4+ T cells constitute both Th2 and Treg populations
Fig 1
Activated CD4+ T cells expand and exhibit Th2 and Treg function in during S. ratti infection.
(A) Gating strategy for CD4+ T cells in the lungs. Dump gate includes B220/CD45R, CD11b, and CD11c. (B) Activation status and transcription factor expression in CD4+ T cells from lungs of S. ratti infected mice 14 days post-initial infection (3 total infections, 1,000 iL3 per infection). (C) Frequency of activated, GATA3+, Foxp3+, and Foxp3+GATA3+ cells in the lungs of S. ratti-infected and naïve mice. (D) Type 2 and regulatory cytokine production by MACS-sorted CD4+ T cells from pooled lymph nodes at 0, 7, and 14 days post-infection following 72 hours of culture in the presence or absence of anti-CD3/CD28 antibody. (E) Total serum IgE and IgG1 levels at 0, 7 and 14 days post-infection. (F) Fecal egg deposition and adult worm counts from wild-type and RAG1-/- mice infected with S. ratti. Significance determined by Mann-Whitney test; *p <0.05, **p < 0.01.