Rapid, reliable, and reproducible cell fusion assay to quantify SARS-Cov-2 spike interaction with hACE2
Fig 1
Soluble proteins inhibit cell fusion in the transient system.
Increasing concentrations of soluble hACE2 (A) and soluble Spike RBD (C) were pre-incubated with spike-expressing cells or hACE2-expressing target cells as in S3 Fig, and RLU quantified the next day. Spike pseudotyping in the presence of soluble hACE2 (B) and soluble Spike RBD (D) was performed using 293T-hACE2 targets, with RLU measured at ~72 h.