Robust CAR-T memory formation and function via hematopoietic stem cell delivery
Fig 5
Costimulatory domain 4-1BB, but not CD28, allows for successful CAR T cell development.
Humanized BLT mice were transplanted with CD34+ cells transduced with either CD4CAR, D1D2CAR, CD4CAR 4-1BB, D1D2CAR 4-1BB, CD4CAR CD28 or D1D2CAR CD28 lentiviruses. A) CAR+% among CD34+ hematopoietic stem cells from bone marrow 20 weeks after engraftment. B) CAR+% among peripheral T cell (CD45+CD2+CD56-). C) CAR+% among peripheral B cell. D) CAR+% among CD5+CD2+TCR- thymocytes. E) CAR+% among CD5+CD2+TCR+ thymocytes. * p<0.01, **p<0.001, ***p<0.0001 by Mann-Whitney test.