ASC-dependent inflammasomes contribute to immunopathology and mortality in herpes simplex encephalitis
Fig 3
IL-1β production is microglia-dependent in an organotypic brain slice culture model of HSV encephalitis.
Microglia were depleted from organotypic brain slice cultures by incubation with clodronate-filled liposomes and IL-1β production was quantified after HSV-1 infection. (A) Diagram of experimental set-up. Slices were allowed to settle for 24 hours in culture before incubation with either clodronate-filled or empty liposomes. Slices were then infected with 106 PFU HSV-1 KOS and culture media was collected at 48 hour post-infection. (B) Microglia quantification by flow cytometry after 6 days of clodronate or empty liposome treatment (n = 4 slices per group). (C) Protein levels of IL-1β as quantified by ELISA (n = 3 slices per group). Values are expressed as means ± SEM (*P < 0.05, **P < 0.01).