Contributions of IFN-γ and granulysin to the clearance of Plasmodium yoelii blood stage
Fig 7
CD8+ T cells and GNLY contribute to P. yoelii infection control.
Comparison of percentage parasitemia in RBCs (A) and Retics (B) in β2-m KO (green line) and WT mice (black line) shows the presence of a second peak of parasitemia around 19 DPI in β2-m KO mice. (C) 20% of β2-m KO mice (green line) die from P. yoelii but all WT mice (black) survive. Comparison of percentage parasitemia in RBCs (D) and Retics (E) in GNLY-Tg mice (orange line) and WT mice (black line) shows earlier control of parasitemia in GNLY-Tg mice compared to WT mice. P. yoelii-infected WT (F and H) and GNLY-Tg (G and I) mice have more activated T cells and higher expression of granzyme B in CD8+ T cells than uninfected mice. GNLY-Tg mice treated with αCD8 have higher parasitemia 8 DPI and delayed parasite clearance (J) and reduced survival (K) compared to control GNLY-Tg mice that received αKLH or PBS. The statistical analysis of parasitemia was performed using two-way ANOVA followed by Bonferroni post-hoc test. The statistical analysis of survival was performed using a log-rank test. Data are pooled from two independent experiments (n = 7–12, for each group). The statistical analysis of T cells was performed using the Kruskal-Wallis test and data are pooled from two-three independent experiments (n = 4–6, uninfected mice; n = 9–12, P. yoelii-infected mice). *p<0.05; **p<0.01; ***p<0.001.