Inhibition of spumavirus gene expression by PHF11
Fig 3
Antiviral activity of mammalian PHF11 proteins: Murine and feline PHF11 genes do not inhibit FV replication.
(A) Western blot analysis of doxycycline inducible human, macaque and mouse myc-PHF11 and LaminB1 loading control in U3-GFP cells. (B) Western blot analysis of doxycycline inducible human and cat myc-PHF11 and LaminB1 loading control in human U3-GFP and feline CRFK cells. (C) Mouse PHF11 genes do not inhibit PFV replication. Spreading replication of PFV (starting MOI = 0.01) in U3-GFP cells expressing doxycycline-inducible myc-tagged human (Hs), macaque (Mac) or mouse (Mmd) PHF11 in the presence (white bars) or absence (black bars) of doxycycline (Dox)pretreatment. Infection was measured via SYBR-Green RT assay. (D) Feline PHF11 does not inhibit PFV or FFV replication. Spreading replication of PFV or FFVFCA (starting MOI = 0.05) in U3-GFP or CRFK cells expressing doxycycline-inducible myc-tagged Hs (circles) or feline (Fc) (diamonds) PHF11 in the presence (open symbols) or absence (filled symbols) of Dox. Representative of at least three independent experiments. Infection was measured via SYBR-Green RT assay. RT, reverse transcriptase. MOI, multiplicity of infection.