The E3/E4 ubiquitin conjugation factor UBE4B interacts with and ubiquitinates the HTLV-1 Tax oncoprotein to promote NF-κB activation
Fig 2
Co-localization of UBE4B and Tax in HTLV-1-transformed cells.
(A) Immunoblotting was performed with the indicated antibodies using cytoplasmic and nuclear fractions obtained from MT-2, HUT-102 and C8166 cells. (B) Immunofluorescence (IF) confocal microscopy was performed using MT-2 and C8166 cells with the indicated antibodies. The fluorescence intensity profile was plotted along the white arrow crossing the nucleus and represented in the graph showing overlap using NIS Element software. (C) PLA was performed using MT-2 and C8166 cells with Tax and UBE4B antibodies. (D) Graphical representation of the number of PLA signals with the secondary probe stained sample as a negative control. Unpaired Student’s t-test, **P <0.01.