Identification of Interleukin1β as an Amplifier of Interferon alpha-induced Antiviral Responses
Fig 2
Difference in mRNA stability despite comparable dose-dependency of the expression profiles of given antiviral genes.
(A) IFNα dose-dependent mRNA expression of antiviral genes. Huh7.5 cells were treated with increasing doses of IFNα for 4 hours. The cells were lysed, total RNA was extracted and analyzed by qRT-PCR. The error bars represent standard deviations (SD) based on biological triplicates. Regression line: sigmoidal four-parameter Hill function; red point: inflection point; dashed line: calculated EC50; a.u.: arbitrary units. (B) Quantification of the mRNA half-lives of the selected antiviral genes. Huh7.5 cells were stimulated with 500 U/ml IFNα for 8 hours and then treated with 5 ng/ml actinomycin D for the indicated times. Total RNA was extracted and analyzed by qRT-PCR. The data points represent biological duplicates. Regression line: three-parameter exponential decay function, dashed line: calculated RNA half-life.