Mycobacterium abscessus infection leads to enhanced production of type 1 interferon and NLRP3 inflammasome activation in murine macrophages via mitochondrial oxidative stress
Fig 7
Crosstalk between IFN-I and IL-1β in MAB-R infection of murine macrophages.
(A) BMDMs were obtained from wild-type (WT) or IFNAR1 KO mice and infected with MAB-R and -S strains at an M.O.I. of 10 for 24 h. Supernatants from the infected cells were collected, and IL-1β and IFN-β cytokine levels were measured by ELISA. (B-C) BMDMs were treated with anti-mouse IgG (10 μg/ml) or anti-mouse IL-1β receptor (10 μg/ml) neutralizing antibodies. Then, the cells were infected with MAB-R and -S stains at an M.O.I. of 10 for 24 h. IL-1β and IFN-β cytokine levels in the infected cell supernatants were measured by ELISA (B), and infected cell lysates were serially diluted and plated onto 7H10 agar plates for CFU assays (C). Error bars represent the SD. Statistical significance was determined by two-tailed Student’s t-test (A-C).