Immunization with a murine cytomegalovirus based vector encoding retrovirus envelope confers strong protection from Friend retrovirus challenge infection
Fig 2
MCMV-specific CD8+ T cell response.
CB6F1 mice were infected once or twice in a three-week interval with 2 × 105 PFU of the indicated MCMV-based vectors. (A) Two weeks after a single immunization, the MCMV-specific CD8+ T cell response was analysed after restimulation with a pool of five MCMV-derived peptides. (B, C) Two weeks or 11 weeks after the first immunization, the MCMV-specific CD8+ T cell response was analysed after restimulation with a pool of four peptides representing the conventional MCMV-epitopes M45985-993, M57816-824, M102446-455 and m14115-23 (B) or with a peptide representing the inflationary MCMV epitope m139419-426 (C). Data were acquired in two (A) or one experiments (B, C), each dot represents one mouse, bars indicate mean values. Statistically significant differences compared to unvaccinated mice are indicated by * (P < 0.05; One Way ANOVA, Holm-Sidak post test).