Klebsiella pneumoniae type VI secretion system-mediated microbial competition is PhoPQ controlled and reactive oxygen species dependent
Fig 13
3D model for K. pneumoniae VgrG4.
(A) The bar represents the VgrG4 sequence and depicts the different parts that were modelled (N-term: residues 1–563, Helix: residues 564–636, Repeat: residues 637–677, C-term: residues 678–739). VgrG4570-899 (teal) containing the DUF2345 domain (612–759) intoxicates bacteria and yeast whereas VgrG4838-899 does not. Monomeric 3D model of VgrG4 with the two regions of antiparallel beta strands (beta meander motif) encircled. (B) Trimeric homology model of VgrG4. The functional part of VgrG4570-899 (teal) starts with the unique helical domains and continues with the beta helical spike formed by 12 strands in each monomer. The separately modelled Helix part is inserted in the estimated location, however, the exact orientation in relation to the rest of the protein is unknown. A PAAR protein from Vibrio cholerae (PDB ID: 4jiv (23) is also shown to indicate the likely interaction site with the PAAR protein found in the VgrG4 locus. (C) Monomeric 3D model of VgrG4 with the two regions of antiparallel beta strands (beta meander motif) encircled.