Dysregulation of M segment gene expression contributes to influenza A virus host restriction
Fig 8
In human cells, avian M2 was expressed at higher levels and localized more strongly to perinuclear bodies than human M2.
A549 and 293T cells were inoculated with the indicated viruses, encoding avian or human M segments, at a MOI of 5 PFU/cell. At 8 hpi cells were stained with anti-M2 (Mab E10; red) and DAPI (blue) and imaged by confocal microscopy. Examples of optical sections are shown, either as merged 2-color images or the red and blue channels alone (shown in grey scale). (A) A549 cells with 63x optical and 3x digital magnification (189x total magnification). (B) 293T cells with 63x optical and 3x digital magnification (189x total magnification). Brightness was adjusted for optimal clarity, with all images treated equally.