Primary infection with dengue or Zika virus does not affect the severity of heterologous secondary infection in macaques
Fig 1
Sampling timeline for each cohort.
Sampling and infection schema for all groups of animals presented in this study. Days where an inoculation was performed or samples were collected to run a test are annotated with a box at the time point relative to either the primary viral infection in the DENV control and ZIKV control groups or relative to the secondary infection in cohorts A-C. Secondary infections in cohorts A and C were with ZIKV while secondary infection in cohort B was with DENV. Asterisks denote when the sampling for an animal/animals differed from the Cohort and is as follows: For cohort A, 321142 was infected with ZIKV 164 days after primary exposure to DENV-3. Cohort B animals were challenged with ZIKV twice before DENV infection. The cohort B timeline is shown relative to secondary ZIKV challenge as opposed to primary ZIKV infection, which occurred 357 days prior to DENV-2 infection. For cohort C, animals were infected with ZIKV in two groups and the timeline begins relative to ZIKV infection of the first group of animals. Animals in the second group were infected with DENV 351 days prior to ZIKV infection. Cohort C animals 752662 and 941637 were infected with DENV 387 days prior to SC inoculation with ZIKV. Serum chemistry panels for negative control animals were analyzed on days -4, 0, 3, 10, 14, 21, and 28, except for 774011 who was not sampled on day 0. Day 5 post-infection CBC tests were not included for negative control animals due to incorrect blood collection. Serum chemistry panels were analyzed for ZIKV control animal 411359 on days 0, 1, 2, 3, 4, 6, and 14 while for ZIKV control animal 912116 they were analyzed on days -6, 2, 5, and 11 relative to ZIKV infection. CBC tests were analyzed for ZIKV control animals on days -4, 0, 1, 2, 3, 4, 6, 7, 8, 9, 14, 21, and 28, except for 912116 for whom these data were not collected on days 2, 4, 7, 8, and 10 relative to ZIKV infection. For animals without day -4 samples, day 0 samples were considered baseline samples and vice versa.