The TLR4 adaptor TRAM controls the phagocytosis of Gram-negative bacteria by interacting with the Rab11-family interacting protein 2
Fig 5
FIP2 is required for phagocytosis.
(A) E. coli phagocytosis in FIP2 silenced human primary macrophages. (B) S. aureus phagocytosis in FIP2 silenced human primary macrophages. (C) E. coli and S. aureus phagosome maturation in the macrophages from Fig 5A and 5B after 15+15 min of stimulation. (D) E. coli phagocytosis in FIP2 silenced THP-1 cells. (E) S. aureus phagocytosis in FIP2 silenced THP-1 cells. Phagocytosis was monitored by 3-D confocal microscopy and presented as mean bacterial count per cell (A-E). (F) E. coli and S. aureus phagocytosis in FIP2 silenced THP-1 cells measured by flow cytometry. (G) Average mean fluorescence intensity (MFI) from, n = 3, independent experiments with mean ± SEM. (H) Phagocytosis of live E. coli or S. aureus in FIP2 siRNA treated THP-1 cells. (I) E. coli or S. aureus phagocytosis in THP-1 cells expressing empty vector (pLVX-Empty) or FIP2 expression vector (pLVX-FIP2) measured by flow cytometry. n = number of cells monitored per condition. One-way ANOVA Kruskal-Wallis test (A, B, D and E) or Holm-Sidak´s test (H) with adj. p values, ** (p< 0.0064), *** (p = 0.0006), **** (p < 0.0001). Red bars: mean ± SD (A-E and H). Data are representative of three or more independent experiments.