Increased Programmed Death-Ligand 1 is an Early Epithelial Cell Response to Helicobacter pylori Infection
Fig 12
Changes in epithelial cell viability in human-derived organoid/immune cell co-cultures infected with H. pylori.
Light micrographs, immunofluorescence staining for E cadherin (E cad, green) and CD8 (red), and flow cytometric dot plots for percentage of Zombie (dead) PD-L1+ve cells in (A-C) control, (D-F) PD-1Inh treated, (G-I) H. pylori (HP) infected, and (J-L) HP+PD-1Inh treated organoid/immune cell co-cultures, and (M-O) PD-1Inh treated organoids and (P-R) HP infected organoids. (S) Flow cytometric analysis for the percentage of PD-L1 positive cells from control, PD-1I, HP infected and HP+PD-1I treated co-cultures. (T) Flow cytometric analysis for the percentage of PD-L1+ve gated Zombie (dead) cells from control, PD-1I, HP infected and HP+PD-1I treated co-cultures. *P<0.05 compared to control group by one-way ANOVA, n = 3 individual organoid experiments.