Increased Programmed Death-Ligand 1 is an Early Epithelial Cell Response to Helicobacter pylori Infection
Fig 11
Organoid/dendritic cell and cytotoxic T lymphocyte co-cultures.
(A) Schematic diagram demonstrating the co-culture of human-derived gastric organoids (huFGOs) and autologous dendritic cells (DCs) and cytotoxic T lymphocytes (CTLs). Representative flow cytometric dot plots of (B) unstained control sample, and (C) HLA-DRhigh dendritic cells. (D) Quantitative flow cytometric analysis for the percentage of live CD8+ve CTLs extracted from co-cultures expressing PD-1, IL-2 and IFNγ. *P<0.05 compared to control group by two-way ANOVA, n = 3 individual organoid experiments. (E) Percentage of CD8+ non-proliferating, low, moderately and high proliferating cells determined by CFSE staining *P<0.05 compared to control group by two-way ANOVA, n = 3 individual organoid experiments, and (F) proliferative index. Quantitative flow cytometric analysis of control or HP infected (G) % live EpCam positive cells, (H) %EpCam Positive cells expressing PD-L1. *P<0.05 compared to control group by one-way ANOVA, n = 3 individual organoid experiments.