Increased Programmed Death-Ligand 1 is an Early Epithelial Cell Response to Helicobacter pylori Infection
Fig 8
Phenotypic characterization of parietal cells in gastric epithelial monolayers and 3D gastric organoids.
Acridine orange accumulation in of gastric epithelial monolayers (A) before, and (B) after histamine stimulation, (C) before and (D) after HP infection. Acridine orange accumulation of 3D gastric organoids (E) before and after histamine stimulation, and (F) after a 24 hour HP infection. (G) Quantification of the ratio shift in F458 (red)/F488 (green) in treated gastric epithelial monolayers during treatment (Histamine) or HP infection (H. pylori), and during histamine treatment of 3D gastric organoids (huFGO). *P<0.05 compared to control group by one-way ANOVA, n = 3 individual cells. Immunofluorescence staining for H. pylori (green), H+/K+ ATPase positive parietal cells (red) and Shh (cyan) in (H) control, or (I) HP infected gastric epithelial monolayers. (J) Western blots for the expression of GAPDH and Shh in control, HP and ΔCagA infected gastric epithelial monolayers.