Increased Programmed Death-Ligand 1 is an Early Epithelial Cell Response to Helicobacter pylori Infection
Fig 5
Changes in PD-L1 expression in FHGOs and AHGOs infected with H. pylori.
Immunofluorescence staining for PD-L1 (green), GSII (red) and TFF2 (blue) in (A) uninfected control, (B) GANT61 treated, (C) H. pylori (HP) infected, (D) HP and GANT61 treated and (E) ΔCagA infected FHGOs. (F) Western blot analysis for the expression of PD-L1 using whole cell lysate collected from each treatment group of the FHGOs and AHGOs. (G) Western blots were quantified using ImageJ. *P<0.05 compared to control groups by one-way ANOVA, n = 3 individual organoid experiments.