Lymph nodes are sites of prolonged bacterial persistence during Mycobacterium tuberculosis infection in macaques
Fig 8
Immune response in thoracic LNs of cynomolgus macaques with granulomas.
Cytokine production in thoracic LN with granuloma in response to ESAT6+CFP10 (A-B) or PDBu and ionomycin (C-E) between LNs with bacterial burden (CFU+, red) and those that cleared (CFU-, blue). A. Frequency of CD11b+ cells producing IL-10 (n = 10 macaques; 27 LNs); B. Frequency of CD4+ T cells producing TNF (n = 24 macaques; 48 LNs). C. Frequency of CD3+ cells producing IFNγ(n = 12 macaques; 35 LNs); D. Frequency of CD8+ cells producing IFNγ(n = 12 macaques, 34 LNs) IL-2 (n = 10 macaques; 28 LNs) and TNF (n = 12 macaques; 34 LNs); E. Frequency of CD20+ cells producing IL-2 (n = 11 macaques; 20 LNs). Each symbol represents a LN. Statistical test is Mann-Whitney.