Xylose donor transport is critical for fungal virulence
Fig 6
UDP-Xyl transport is required for host interactions and virulence.
(A) Percent phagocytosis (engulfed fungi/initial inoculum) of opsonized fungi. (B) Fold-change in colony-forming units (CFU) 24 h:0 h after internalization. (C) Proportion of daughter cells in the population of WT (dashed line) and uxt1Δ uxt2Δ (black line) cells incubated with BMMs for 0, 24, and 48 h. Data are the mean ± SEM of three independent experiments. *, p < 0.05 by (A, B) one-way ANOVA with Tukey’s post hoc test or (C) Student t-test. (D) Survival of A/JCr mice after intranasal inoculation with 5 × 104 cells of the indicated strains (n = 8–9). (E) Lung CFU of infected mice at the time of death (for WT, uxt1Δ, uxt2Δ, and complemented mutants; n = 8) or at the indicated time points (for uxt1Δ uxt2Δ; n = 3). Open circles, individual mice; black bar, mean; dashed line, initial inoculum. **, p < 0.01 by one-way ANOVA with Tukey’s post hoc test.