Diminished neutrophil extracellular trap (NET) formation is a novel innate immune deficiency induced by acute ethanol exposure in polymicrobial sepsis, which can be rescued by CXCL1
Fig 9
rCXCL1 augments extracellular bacterial killing of neutrophils.
A-B, Alcohol impairs the extracellular bacterial killing activity of mouse bone marrow-derived neutrophils which can be improved by rCXCL1. Bacterial killing of mouse neutrophils treated with alcohol (25 mM) prior to infection (MOI 1) was determined by assessing extracellular CFUs at 60 min after infection with E. coli (A) or S. aureus (B). Experiments were performed in triplicate wells and 3–5 mice/group were used (*, p <0.05).