Multiple UBXN family members inhibit retrovirus and lentivirus production and canonical NFκΒ signaling by stabilizing IκBα
Fig 2
Characterization of UBXN1 functional domains by reporter assay.
(A) Quantification of NFκB-FFLUC reporter in HEK293 cells 48 h after 96-well format transfection with indicated Myc-UBXN1 constructs (80 ng per well) or empty plasmid (Vector), in the presence 5 ng/mL TNFα for 4h; (B) Similar to (A), using an HIV LTR-FFLUC reporter; (C) Similar to (A), using an AP1-FFLUC reporter. All data represent mean ± SEM (n = 3). ****p < 0.0001, ***p < 0.0005, **p < 0.005, *p < 0.05, compared to vector alone, by two-way ANOVA.