How Quorum Sensing Connects Sporulation to Necrotrophism in Bacillus thuringiensis
Fig 5
The same key residues are involved in the two functions of NprR.
(A) Effect of NprR mutations in sporulation efficiency in the Bt ΔRX strain. The number of viable bacteria (blue bars) and heat-resistant spores (orange bars) was counted after 3 days of growth in LB medium at 30°C. The percentage of sporulation was indicated at the top of histogram bars. Error bars: Standard Error of the Mean (SEM). The experimental values are given in S1 Table. (B) Close view of the peptide-binding site in the NprR-NprX complex (PDB ID 4GPK). The bound peptide is shown as sticks coloured by atom type. Its K residue is labelled and highlighted by dots as well as NprR residues H201, W167, Y165 and R343. (C) Close view of the peptide-binding site in the NprR-apoB conformation. The increased distance between the NprR residues Y165 and R343 is indicated.