A Putative Small Solute Transporter Is Responsible for the Secretion of G377 and TRAP-Containing Secretory Vesicles during Plasmodium Gamete Egress and Sporozoite Motility
Fig 6
FRAP reveals dynamic mobility of membrane-bound PAT::GFP.
(A) Plot profile along the red lines confirms membrane localization of PAT::GFP at the rear of the sporozoite (i) and the dominant microneme localization at the front of the sporozoite (ii) (B) Quantification of GFP signal recovery after FRAP at the membrane (i) and micronemes (ii), indicated in the red squares. (C) Representative images of sporozoites expressing PAT::GFP during FRAP at the membrane (i) and micronemes (ii). (D) Micronemal SS::GFP::TRAP does not recover after photobleaching. Arrows indicate direction of movement. All scale bars: 5μm.