Type I Interferon Receptor Deficiency in Dendritic Cells Facilitates Systemic Murine Norovirus Persistence Despite Enhanced Adaptive Immunity
Fig 7
CW3-specific CD8 T cells are functional during persistent infection.
Cells were collected from spleens of CD11c-Ifnar1-/- mice and littermate controls 21 days after infection with CW3. The number and percentage of CD8 T cells that were tetramer positive (A) or responded to ex vivo peptide stimulation (B) were determined as in Fig 4. (C) RNA extracted from spleens was used to quantitate transcripts for Ifng, Tnfa, and Gzmb. (D) The percentage of cells in (B) positive for one, two, or three of the markers of activation is shown. (E) Tetramer positive CD8 T cells in the spleens of CD11c-Ifnar1-/- (red lines) and littermate controls (blue lines) were analyzed for cell surface expression of Ly6C, CD103, and PD-1. Grey lines are histograms of total CD8+ T cells. Dot plots show the geometric MFI for each marker. Data in (E) is representative of two independent experiments. Data in (A-D) is combined from two (C) or three (A, B, D) experiments with individual mice shown in (A-C) and 13 mice per data point in (D). Statistical significance was determined by unpaired t test (A, B, C) or 2-way ANOVA (D). n.s = p>0.05, * = p≤0.05, ** = p≤0.01, *** = p≤0.001, **** = p≤0.0001.