Role of Ultraviolet Radiation in Papillomavirus-Induced Disease
Fig 4
Systemic effect of UVB assists in MmuPV-dependent papillomatosis.
(A) Schematic illustrating the experimental design. Twenty-four hours post infection with 108 VGE, groups of mice were either whole body UVB irradiated (FVB+MmuPV+UVB 24h.p.i.) or infected sites were shielded from UVB (FVB+MmuPV+UVB 24h.p.i. shielded) and scored weekly for presence of ear papillomas up to 6 months. UVB dose was 300mJ/cm2. Scoring data is compared to the FVB+MmuPV+UVB 24h.p.i. group also shown in Fig 2, as both experiments were performed at the same time. (B) Kaplan-Meyer plots of the fraction of papilloma-free infected sites over the first 12 weeks post-infection. There was no significant difference between the two experimental groups (shielded versus unshielded ears) (P = 0.938) as assessed by Wilcoxon log-rank analysis. (C) Percentage of sites with overt papillomas over a 6-month observation period. Some papillomas completely regressed in both groups. Control animals i.e. non-UVB irradiated FVB mice infected with MmuPV1 (FVB+MmuPV-UVB) did not develop any papillomas over the 6 month observation period.