Role of Ultraviolet Radiation in Papillomavirus-Induced Disease
Fig 3
Histopathological analysis of MmuPV1-induced papillomas in UVB –irradiated mice.
(A) H&E staining of ear tissue indicating presence of a sessile ear papilloma caused by MmuPV1 in FVB mice following UVB irradiation (left). Presence of koilocytes is indicated (middle). L1-immunofluorescent staining of papillomas (right). (B) Sessile papilloma with areas indicating frank malignant transformation (inset;i) and foci of inflammation (inset;ii). K14 staining of papilloma and associated epithelial invasion into underlying dermis. Papillomas on the tail of an immunodeficient BALB/c FoxN1nu mice (right, top). These papillomas were formed as a result of infection with viral extracts prepared from papillomas that arose in immunocompetent FVB mice infected with MmuPV1 followed by UVB irradiation. (C) L1 (green)-MmuPV1 FISH (red) co-staining of MmuPV1-induced ear papillomas in UVB irradiated FVB/NJ mice. The nuclei were counterstained with DAPI (blue). Scale bars represent 100μm.