The Combined Deficiency of Immunoproteasome Subunits Affects Both the Magnitude and Quality of Pathogen- and Genetic Vaccination-Induced CD8+ T Cell Responses to the Human Protozoan Parasite Trypanosoma cruzi
Fig 3
Mice lacking immunoproteasomes have diminished expression of MHC class I molecules and reduced numbers of CD8+ T cells.
WT and TKO mice were infected s.c. with 104 T. cruzi parasites (Y strain) or left uninfected. Twenty days later, spleen cells were collected and stained for surface markers. (a) gMFI (geometric mean of fluorescence intensity) of H-2Kb and (b) I-Ab stainings are shown after gating in CD11c+ I-Ab+ CD3- CD19- cells. Total numbers of CD8+ and CD4+ T cells in the spleen of these animals are shown in (c) and (d), respectively. Results are shown as individual values and as the mean ± SEM for each group (n = 3). One representative of at least two independent experiments is shown. Asterisks indicate that the values observed for TKO mice were significantly different than those for WT mice (*P<0.05 **P<0.01 ***P<0.001).