Interaction of NCOR/SMRT Repressor Complexes with Papillomavirus E8^E2C Proteins Inhibits Viral Replication
Fig 7
HPV 8 E8^E2C recruits NCoR/SMRT complexes to distinct nuclear foci.
RTS3b were transfected with a URR reporter plasmid for HPV8 and with expression vectors for E1 (500 ng) (pCMV neo 3xFlag-8E1), E2 (50 ng) (pSG8 E2) and E8^E2C or the E8^E2C KLK mt proteins (50 ng) (pSG 8 E8^E2C HA or pSG 8 E8^E2C KLK mt HA). Cells were stained with the indicated primary antibodies and analyzed by immunofluorescence microscopy. DNA was stained with DAPI (blue). Signals for E8^E2C (or the E8^E2C KLK mt) and NCoR/SMRT complex components in foci from 6–9 individual cells were analyzed for colocalization using optical sections and Zeiss Axiovision40 4.8.2 software. Colocalization is expressed as Pearson´s correlation coefficient. Statistical significance between wt and mt was determined by Students t-test (**p<0.01; ***p<0.001).